| Route | Brescia – Mantua – Cremona – Brescia |
| Date | 28.04.1940 |
| Laps | 9 |
| Distance | Apross. 1.485,000 km / 922,933 miles |
| Time | 8 hours, 54 minutes, 46 seconds |
Due to the painful aftermath of the Mille Miglia accident in 1938, this race took place on a set 165 km triangular route from Brescia via Mantua and Cremona back to Brescia.
The first cars built by Enzo Ferrari , the AAC 815s, made their debut appearance in this race.
| No | Driver | Ferrari | S/N | Team | Result |
| 65 | Marchese Lotario Rangoni Macchiavelli Enrico Nardi |
AAC 815 | 020 | Marchese Lotario Rangoni Macchiavelli | retired |
| 66 | Alberto Ascari Giuseppe Minozzi |
AAC 815 | 021 | Alberto Ascari | retired |